Safranin is a red color cationic dye. It is mostly used in plant histology protocols to stain the nuclei in red and some cell walls, such as secondary cell walls, where lignin is stained. It is also useful for Gram staining in bacteria.
Procedure
1 g safranin (C.I. 75100)
15,5 ml 95º ethanol
14,5 ml distilled water
Notes
Dilute the safranin solution with 50º ethanol (1:1) before used.
Products
Distilled water
Safranin (C.I. 75100)
95º ethanol
50º ethanol
Labware
Test tube
Balance
Magnetic stirrer
Flasks
Bottles