Carazzi's hematoxylin is a good dye to stain nuclei, and with few background staining. It can be used for general staining, combined with eosin. Carazzi's hematoxylin staining is a progressive staining.
Components
800 ml distilled water
200 ml glycerol
1g hematoxylin (C.I. 75290)
0,2g Sodium Iodate
50 g Potassium alum (KAl(SO4)2·12H2O)
Procedure
Mix hematoxylin and glycerol. Solve the sodium iodate in a bit of water and dissolve the potassium alum in the remaining water. Mix the hematoxylin-glycerol and the potassium alum, and then slowly add the sodium iodate. Filter the final solution.
The solution can be used immediately, and it is stable for months.
Notes
It is a progressive statining, with low background.
In frozen sections, a double staining is recommended.
Products
Hematoxylin (C.I. 75290)
Glycerol
Sodium iodate (NaIO3)
Potassium alum (potassium aluminium sulfate: KAl(SO4)2·12H2O)
Distilled water
Labware
Test tube
Balance
Magnetic stirrer
Flasks
Dark bottles